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| Franco,Maurício Machaim; Antunes,Robson Carlos; Oliveira,Katiana Mello de; Pereira,Cícero Donizete; Biase,Fernando Henrique; Nunes,Francis de Morais Franco; Goulart,Luiz Ricardo. |
| Fourty-six non-castrated, halothane-free, male Landrace pigs were genotyped by PCR-RFLP for the Rsa I polymorphism in the PIT1 gene and classified into AA and AB genotypes. Total RNA was extracted from the pituitaries and the relative quantities of growth hormone (GH) mRNA were determined by semi-quantitative RT-PCR. Pigs with the AB genotype had higher levels of GH mRNA than those with the AA genotype (p = 0.034; Kruskal-Wallis test). This result suggests that the Rsa I polymorphism may be involved in Pit-1 protein expression or function, which in turn may influence GH transcription and expression. Thus, the Rsa I PIT1 gene polymorphism in this pig line may be used as a molecular marker to identify higher GH expression and possibly select for carcass and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PIT1 gene; Growth hormone mRNA; Semi-quantitative RT-PCR; Pig. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100003 |
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| Franco,Maurício Machaim; Almeida,Juliana Franco; Souza,Guilherme Rocha Lino de; Antunes,Robson Carlos; Goulart,Luiz Ricardo. |
| After the advent of the genome projects, followed by the discovery of DNA polymorphisms, basic understanding of gene expression is the next focus to explain the association between polymorphisms and the level of gene expression, as well as to demonstrate the interaction among genes. Among the various techniques for the investigation of transcriptional profiling involving patterns of gene expression, quantitative PCR is the simplest analytical laboratory technique. The objective of this work was to analyze two strategies of a competitive PCR technique for the quantification of the pig growth hormone (GH) gene expression. A pair of primers was designed targeting exons 3 and 5, and two competitive PCR strategies were performed, one utilizing a specific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pig; GH gene; Gene expression; RT-PCR. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100003 |
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| Alonso,Vivian; Santana,Bárbara Amélia Aparecida; Pirage Junior,Waldesse; Goulart,Luiz Ricardo; Diniz,Heyder da Silva; Machaim,Maurício Franco; Borges,Graciele Segantini Nascimento. |
| O aumento da produtividade e qualidade dos produtos animais vem se tornando de grande interesse econômico. A prolactina (PRL) é um hormônio essencial para o sucesso reprodutivo e seu receptor (RPRL) tem sido detectado em vários tecidos². O gene RPRL foi recentemente mapeado em suínos no cromossomo 16(6). Este trabalho teve como objetivo analisar a frequência genotípica do RPRL em três diferentes raças de suíno, Landrace, Large White e Pietrain e correlacionar os genótipos com características de interesse. Foram analisados um total de 124 animais. O DNA foi extraído de sangue total suíno e submetido a técnica de PCR-RFLP, para determinação do genótipo do gene do receptor da prolactina. As análises estatísticas mostraram que o genótipo RPRL teve efeito sobre... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prolactina; Receptores; Genes; Suínos. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000500008 |
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| Cardoso-Júnior,Carlos A.M.; Fujimura,Patrícia Tieme; Santos-Júnior,Célio Dias; Borges,Naiara Araújo; Ueira-Vieira,Carlos; Hartfelder,Klaus; Goulart,Luiz Ricardo; Bonetti,Ana Maria. |
| Abstract Stingless bees of the genus Melipona, have long been considered an enigmatic case among social insects for their mode of caste determination, where in addition to larval food type and quantity, the genotype also has a saying, as proposed over 50 years ago by Warwick E. Kerr. Several attempts have since tried to test his Mendelian two-loci/two-alleles segregation hypothesis, but only recently a single gene crucial for sex determination in bees was evidenced to be sex-specifically spliced and also caste-specifically expressed in a Melipona species. Since alternative splicing is frequently associated with epigenetic marks, and the epigenetic status plays a major role in setting the caste phenotype in the honey bee, we investigated here epigenetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA methylation; Histone modification; Caste development; Social bees; Genetic caste determination. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100061 |
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| Oliveira,Rosana de Cássia; Nunes,Francis de Morais Franco; Campos,Ana Paula Soraggi; Vasconcelos,Soraya Matos de; Roubik,David; Goulart,Luiz Ricardo; Kerr,Warwick Estevam. |
| One of the commonest neotropical stingless bees is Tetragonisca angustula (Latreille, 1811), popularly known in Portuguese as jataí, which occurs in variable nesting sites from Mexico to Argentina. We used 18 primers to generate 218 RAPD markers which we used to determined the genetic distance between T. angustula populations from 25 localities in three different Latin America countries, using Tetragonisca weyrauchi from the Brazilian state of Acre and the common honey bee (Apis mellifera) as outgroups. Genetic distance, calculated as the Percentage of Dissimilarity (14%), based on all markers divided the T. angustula population into eastern (group 1) and western (group 2) groups. However, we were able to separate the two groups by using only two primers... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tetragonisca angustula; Genetic divergence; Molecular markers; UPGMA. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200009 |
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| Borges,Graciele Segantini do Nascimento; Goulart,Luiz Ricardo. |
| Genotype data of 477 animals of several swine races (Landrace - LD, Large White - LW, Pietrain - PI, LWXLDXPI, Piau, Monteiro, and unknown race) were obtained to determine the allele frequency of the obesity gene. Genotype data of 174 crossbred swine (LWXLDXPI) were also obtained, in order to assess its correlation with carcass evaluation data (lean meat percentage, backfat thickness at P2, loin eye area, adjacent fat area, total fat and meat). Finally, genotype data of 96 pure swine (Landrace, Large White and Pietrain) were collected, to establish its relation with meat quality (drip loss, meat color, texture analysis and intramuscular fat) and carcass evaluation data (lean meat percentage; ham, loin, shoulder and belly weights; and backfat thickness at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Obese gene; Leptin gene; Quantitative traits. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100007 |
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| Biase,Fernando Henrique; Franco,Maurício Machaim; Goulart,Luiz Ricardo; Antunes,Robson Carlos. |
| Molecular diagnostics are performed by using DNA from different body tissues. However, it is necessary to obtain genomic DNA of good quality. Due to the impossibility of collecting blood from slaughtered animals, DNA extraction from solid tissues is necessary. The objective of this study was to describe a protocol of DNA extraction from swine skin, adipose, brain, liver, kidney and muscle tissues. We obtained high molecular weight DNA of good quality, shown by agarose gel and amplification of two DNA fragments, 605bp and 891pb, by PCR. Spectrophotometric analysis of DNA concentration showed variation among the DNA from different tissues, with the liver and adipose tissues presenting the greatest and the smallest concentration, respectively. The described... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: DNA extraction; Swine tissues. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300011 |
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